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Sperm subpopulations rat

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    Aida M. The aim of this study was to use computer-assisted sperm analysis CASA to examine changes in motion parameters of rat spermatozoa incubated under culture conditions that support IVF. Rat cauda epididymal spermatozoa were evaluated in six replicate experiments, at 0 and 4h of incubation. Visual inspection of sperm tracks after 4 h of incubation revealed classical hyperactivation patterns. Bivariate models were evaluated to objectively define the subpopulation of hyperactivated HA spermatozoa. The efficacy of detecting HA spermatozoa was evaluated using sperm tracks that were visually classified as HA or progressive.
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    Automated analysis of toxicant-induced changes in rat sperm head morphometry

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    Automated analysis of toxicant-induced changes in rat sperm head morphometry

    The aim of this study was to compare the post-thaw distribution of motile sperm subpopulations, following simple or colloid centrifugation. A new analysis was used to evaluate the available number of sperm from each subpopulation after each centrifugation protocol. Percentage of total and progressive motility TM and PM , as well as sperm motility kinematics, distribution of motile sperm subpopulations, and recovery rates, were statistically compared among treatments. Following each SLC procedure, different percentages of the subpopulation with the most vigorous and progressive sperm sP4 were obtained. From a practical point of view, sperm washing, the standard centrifugation procedure for equine semen processing, recovered the same amount of fast and progressive sperm as colloid centrifugation, apparently the best treatment according to traditional analysis.
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    The aim of this study was to compare the post-thaw distribution of motile sperm subpopulations, following simple or colloid centrifugation. A new analysis was used to evaluate the available number of sperm from each subpopulation after each centrifugation protocol. Percentage of total and progressive motility TM and PM , as well as sperm motility kinematics, distribution of motile sperm subpopulations, and recovery rates, were statistically compared among treatments.
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